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Sequencing - (Jan/14/2008 )


I m trying to sequence a gene (2530bp), but after sequencing I found to mutch NNNNNN and I can't get a good sequence.
Can some one kindly tell me what is the problem?


-Marzougui salem-

Talk to your sequencing people. It could be many things, such as poor quality DNA, multiple binding locations for the primer, too much DNA, too little DNA, presence of inhibitors in water, you name it. Have you successfully sequenced other things?


As suggested, consult the sequencing center. They can help trouble shoot.