how to prepare EDTA wash - (Jan/10/2008 )
QUOTE (phage434 @ Jan 15 2008, 07:07 AM)
EDTA will not dissolve until NaOH is added in large amounts (approximately equimolar). It's been a very long time since I've tried, but I recall that it is impossible to make a 1 M solution, which is why the stock solution is 500 mM.
yes phage you're right, the solution stayed blank until I've added NaOH (20g per liter).
Thanks for the help
-SMH-
QUOTE (Dominic @ Jan 15 2008, 04:46 AM)
QUOTE (mdfenko @ Jan 14 2008, 09:10 PM)
QUOTE (Dominic @ Jan 11 2008, 11:40 AM)
i tend to think of edta as soapy water
are you thinking of sds?
no
i use it for epitope retieval (i suppose its a different kind of soap to sds - or am i completely deluded in this matter?)
maybe detergent would be a better word
edta is neither a soap nor a detergent. it is not a surfactant, it is a chelator (it is found in shampoos and other soaps and detergents to "soften" the wash water).
-mdfenko-
i'm just going to give up in this thread
btw it was 1mM not 1M (sorry)
-Dominic-