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Is this gene going to express? - is only the firs ATG read and express or all 3 reading frame? (Jan/10/2008 )

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QUOTE (aztecan princess @ Jan 11 2008, 04:42 PM)
Other Question…. We are cloning viral genes for expression like this gene. This genes don’t have a native kozak sequence. Do you think we would add it to have a better expression, or they would be expressed without problem?

If the virus use the cell‘s machinery (eucariotic) to express their genes in nature, I think the kozak sequence won’t be necessary for express the same genes in an expression vector. But I think may be adding kozak sequence would increase the expression level.

What do you think????????

In our viral vectors, we always add the kozak sequence for better expression.


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