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Mouse embryo sections - how to see optic stalk? - (Jan/04/2008 )

Dear all,

I am trying to make some sections with mouse embryos between E10.5 to E14.5. The embryos are X-Gal stained (wholemount) and then paraffin-embedded. I want to see the optic stalk and the eye together, I tried both cross sections and sagittal sections but so far I couldn't succeed to see any optic stalk at all, the eye part is OK. Does anyone have any suggestions for the embedding and sectioning steps?

thanks in advance!

-toprak-

QUOTE (toprak @ Jan 4 2008, 01:48 PM)
Dear all,

I am trying to make some sections with mouse embryos between E10.5 to E14.5. The embryos are X-Gal stained (wholemount) and then paraffin-embedded. I want to see the optic stalk and the eye together, I tried both cross sections and sagittal sections but so far I couldn't succeed to see any optic stalk at all, the eye part is OK. Does anyone have any suggestions for the embedding and sectioning steps?

thanks in advance!


I just had a quick look on google and I saw some nice pictures of optic stalk and the developing eye using transverse sections. Not sure if this helps!
How thick are your serial sections?

-Clare-

yes, i saw lots of nice eye sections with optic stalk! i'm making sections at 7, 8, 9 microns. it's really frustrating.

-toprak-

QUOTE (toprak @ Jan 5 2008, 08:47 PM)
yes, i saw lots of nice eye sections with optic stalk! i'm making sections at 7, 8, 9 microns. it's really frustrating.


The sections I saw online had 1 micron thickness....!!!

-Clare-