Promoter studies - (Jan/03/2008 )

Hi Guys

Its me again,
I have cloned the promoter of my gene. is there any way to know if someone has cloned and published it before . Is it possible to publish the f promoter structure like TSS , or important transcription factors or where TATA box is ?? etc.

thanks in advance.

regards
Vani

-vani.khare-

What organism are you working on, human, mouse? Even you have fully characterize the promoter experimentally chances are low to publish in good journals. Characterizing a promoter nowadays is not something that pays off well. You have to do experiments such as footprinting, gel shifting, primer extension, ChIP assay, many of them require radioactive work.

-pcrman-

QUOTE (pcrman @ Jan 3 2008, 08:54 PM)

Thanks PCRman for responding to my quest.......Actually I m done with EMSA and m in the middle of transiant transfection using this promoter . but I just wanted to know if structure of promoter(bioinformatics) can also go in a paper together with other results. And how to know that this promoter is not analyzed before ??
I mean is there any site where they submit promoter sequence or some database?? my promoter is from Zebrafish.

cheers
Vani

-vani.khare-

the zebrafish genome has been published. Go to UCSC genome browser or ENSEMBL to look it up.
The sequence of everything is published in database.

-mikew-