influence of IgL chain on polyreactive Ig heavy chain - (Jan/03/2008 )
To determine if there is a difference between complete IgGs and antibodies that do not express IgL chains, 41 already described polyreactive antibodies from three healthy donors [Tiller, 2007] were re-expressed as complete antibodies and as IgG without IgL chain. In an ELISA assay the antibodies were tested against structurally different antigens. Both immunoglobulins displayed the same reactivity and were ascertained as poylreactive, no detectable differences could be observed in the binding of antigens. The non-existance of the IgL chains does not alter the reactivity of the polyreactive antibodies, there is no detectable silencing of reactive IgH chains due to light chains.
That's what I found out, but I thought that IgL chains would silence IgH chains of B cells expressing polyreactive IgH and are in the periphery. When I understood it correctly, polyreactive B cells in the periphery escaped receptor editing or deletion. Can anybody help me understanding why my data suggest that the light chain does not alter the reactivity of polyreactive IgH chains?
Answer is very simple. In an Antibody, variable region present in either the light chain or heavy chain which has Hyper variable regions that are responsible for their specificity. Since these hyper variable regions are also present in your heavy chain, which enable light chain deleted IgG to recognize the antigens
I ALWAYS WONDER, WHAT IS THE NECESSITY OF ANTIBODY LIGHT CHAINS IN HUMAN PHYSIOLOGICAL CONDITIONS, ARE THERE ANY SPECIFIC FUNCTIONS EXIST???
I would be very hapy if some one can explain the phenomenon...
thanks in advance
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