DITHIOTHREITOL - (Jul/09/2004 )

Hi,
Please tell me the role of DITHIOTHREITOL in PCR.

Thanks

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check this paper

Biochem Mol Biol Int. 1998 Jan;44(1):157-63.
Additive effects of bovine serum albumin, dithiothreitol, and glycerol on PCR.

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Thanks Green.
I try to find the article you mentioned.

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Dear green,
I couldn't find the article. would you please tell me another related article or alternatives?

thanks,
HN1000

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Help again!
I want to know or find detais about Dithiothreitol=DTT and its effect on PCR.
Any articles, online or paper?

thanks

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DTT (dithiothreitol) is used to reduce disulfide bonds quantitatively and maintain monothiols in the reduced state. At low concentrations, DTT stabilizes enzymes and other proteins which possess free sulfhydryl groups and has been shown to restore activity lost by oxidation of these groups in vitro.

DTT is mainly used as a stablizer in PCR. As you can find that many taq polymerases are in DTT containing buffer.

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Dear kawaka,
Thank you very much for your useful information.
But I try to find an article which compare PCR with and without DTT. If you know please point me to that article.

HN1000

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