Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

disappear protein band after development - (Dec/19/2007 )

Pages: Previous 1 2 

QUOTE (mdfenko @ Dec 22 2007, 12:36 AM)
i should have asked this before: did you see the nonspecific bands staining on the first western?

can you try stripping and restaining the first blot?

Hi Mdfenko,

I also see the nonspecific ones on my 1st western. Actually, they are nearly all the same from 1st western till the last one yesterday, except there is no target band only...... wacko.gif



have you tried with a fresh aliquot of antibody?

while i'm at it: have you tried without the first antibody (see if non-specific binding is the secondary)?


Pages: Previous 1 2