Question about phenol/chloroform/isoamyl - (Dec/07/2007 )
I saw someone recommended a while back that we can use the Fishersci PCI, BP1752I-400, to extract DNA. The description of the product is 25:24:1 Mixture, pH 6.7/8.0, Liq. Does that mean the liquid in the vial is already at pH8.0? I know some vial will come at pH6.7, such as the one from Sigma. You need to add the buffer that comes with it to bring the pH to 8.0. I didn't find such a buffer when the Fishersci 25:24:1 BP1752I-400 came. So does this mean the 25:24:1 "mixture" is at pH6.7? But the "liquid" itself is already at pH8.0 AND can be used directly for extracting DNA? Thanks so much for the help.
-ywhsu-
QUOTE (ywhsu @ Dec 7 2007, 05:58 PM)
I saw someone recommended a while back that we can use the Fishersci PCI, BP1752I-400, to extract DNA. The description of the product is 25:24:1 Mixture, pH 6.7/8.0, Liq. Does that mean the liquid in the vial is already at pH8.0? I know some vial will come at pH6.7, such as the one from Sigma. You need to add the buffer that comes with it to bring the pH to 8.0. I didn't find such a buffer when the Fishersci 25:24:1 BP1752I-400 came. So does this mean the 25:24:1 "mixture" is at pH6.7? But the "liquid" itself is already at pH8.0 AND can be used directly for extracting DNA? Thanks so much for the help.
Do you have a pH paper handy?
-genehunter-1-