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2D-PAGE MARKER TROUBLE - (Nov/15/2007 )

i am working on cell lysate proteins of sertoli cells. i am studying proteins by 2D-PAGE. FOR identifying mol.wt. of my proteins, i used bio-rad protein marker. but it is giving smear, no discrete bands in my gel. while my proteins are giving discrete spots after IFE. AM i supposed to use some different marker for gels stained with silver staining after IEF? please tell me if there is some specific marker for 2D-PAGE? waiting for reply.


you probably used too much marker for silver stain. try using much less or, better yet, use markers designed for use with silver stain.