Protein will stripped by overwahsing? - (Oct/31/2007 )
J Immunol Methods. 1989 Aug 15;122(1):129-35
Interesting. I didn't know.
Glad to be of service
Sometimes we have blocked overnight at 4ºC. I don't think it will strip the membrane
We have blocked with 1% milk without differences in background signal of higher percentage of milk. If the background is too high I try other reagent to block at same percentage, such as BSA.