I am facing problem in intravenous injection in mice tail vein please advice - (Oct/26/2007 )

I am trying to make intravenous injection of cells into mice tail vein , the problem that i feel am in but after 50ul injection the vein is blocked , please advice.

are you sure you are in the vein? you could train by injecting blue evans and see if mouse become blue

First be sure you are injecting in the vein. If there is any flow resistance, then you are not in the vein. After finding the vein, you should'nt move the needle so it doesn't get out or perfurates the other side. And try to inject close to the end of the tail so that if you can't inject all you can go up the vein until the base of it.
I guess you're warming them before you inject them! I do it placing a infrared lamp over the cage and it works really well.
Good luck

You can inject up to 400 ul in volume. Some crazy procedures allow you to do 1.5 ml. Best to heat the mouse for 2-3 min with a 200 W lamp to dilate the tail vein before injection. You should not feel any resistance if you insert the needle right into the vein.

hi,
before injecting any solution in to the vein, try to suck little bit of blood carefully (the high low pressure in the syringe can damage the vessel) if you see a tinge of blood in ur syringe then slowly go ahead injecting your sample without moving the needle.

gud luk
sravan payeli