Dual Luciferase assay - What does pRLTK have? (Oct/16/2007 )

Hello,

I am quite new to the filed of miRNA. I want to inhibit my miRNA using antisense oligonucleotide and want to chekc the efficiency of the inhibiton by transfecting pMIR-REPORT vercot with the tragte gene. Also, I want to use pRLTK plasmid(Rennila) for normalization of the luciefarase expression. Is pRLTK an empty vector? So, along with transfecting your luciefrsse reporter you transfect this empty vecto(pRLTK) and then see the expression of both and further normalize it. Is is correct?

Thank you in advance to anyone who can clear this doubt.

maggie

Hi Maggie,

pRL vectors are a family of wildtype Renilla luciferase (Rluc) control reporter vectors. The pRL Vectors provide constitutive expression of Renilla luciferase. They are usually used in combination with a firefly luciferase vector (like pGL3 etc) to cotransfect mammalian cells. Expression of Renilla luciferase provides an internal control value to which expression of the experimental firefly luciferase reporter gene may be normalized.

A resource for further information: http://www.promega.com/catalog/catalogprod...eporter+Vectors

If the link doesn't work, go to promega.com and search for pRLTK.

Hope this helps....

Good luck!