plasmid went missing - what happen?? (May/12/2004 )

I did my plasmid miniprep and did also RE digestion to check my insert.
However, after sending it to sequencing...the plasmid went missing...
I check all my samples and it is what that happened...
what went wrong??

-cetok-

QUOTE

after sending it to sequencing...the plasmid went missing

What do you mean by "the plasmid went missing"? What primer did you use for sequencing?

-rassen-

i use m13f & r primer for sequencing..
anyway, i run gel on my samples..n it's true..
i can't find my plasmid n insert...
that's why i said it's misssing...hehehe

-cetok-

Cetok

Did you use DNaseI to suspend your samplesby mistake? I am sure you are joking or are a really really tired lab worker- go get some sleep

-phdconsult-

well...i'm sure i didn't put in DNase...but only Rnase , as i have no Dnase reagent... anyway, i think this topic should be closed by now..
so please no more replies....thks for looking into my question...sweet of u all...

-cetok-