Extraction protocol to visualize microtubules - (Sep/26/2007 )
Hello,
I´ve heard of a protocol using phalloidin (a drug that polymerizes microfilaments) to help preserve microfilaments during extraction. I was wondering if anyone had already used a drug such as taxol (polymerizes microtubules) to help preserve microtubules during extraction? Does it work?
-Masterofpipets-
i've used taxol to displace maps from microtubules. the tubules form without the maps and then you separate them from each other.
-mdfenko-