cDNA quantification using Nanodrop - (Sep/24/2007 )
I don't have a lot of experience using Nanodrop in quantifying my RNA/DNA, but I don't have a lot of problem with it until I tried to quantify cDNA.
So... can you quantify cDNA using nanodrop?
well treat RNAse and purify to eliminaite remaining RNA and nucleotides, then measure with nanodrop. Ncleotides drives very bad measuremnets.
Thank you for that! I had that idea before, but noone in my lab agreed that it is feasible. I'll give it a go, though!
I´ve always thought that it´s impossible to measure cDNA, at least you can´t be sure of your measurments?! Isn´t it so?!
Why would it be impossible?
I thought I measured my cDNA once... so the concentration I got then is wrong?
Be sure to choose the right kind of nucleic acid you are trying to measure. Somewhere on the screen there is a box for this. I can't remember offhand all the selections available, but I think single-stranded DNA is one of them.