problems with microsatellite sequences - (Sep/11/2007 )
I cloned my pcr product containing the repeat sequences (hopefully) using the Qiagen kit and obtained both forward and reverse sequences using the universal M13 primers. the problem now is i cannot find the microsatellite in both sequences. in other cases i cannot even find my pcr primers, not the M13. isnt this unusual, i was expecting to find the SSR and both the forward and reverse primers.
or how does it work, help!
-sathman-
QUOTE (sathman @ Sep 11 2007, 08:04 PM)
I cloned my pcr product containing the repeat sequences (hopefully) using the Qiagen kit and obtained both forward and reverse sequences using the universal M13 primers. the problem now is i cannot find the microsatellite in both sequences. in other cases i cannot even find my pcr primers, not the M13. isnt this unusual, i was expecting to find the SSR and both the forward and reverse primers.
or how does it work, help!
or how does it work, help!
It depends often on what microsatellite detection/enrichment protocol you use?
-hobglobin-