Why my RNA absorbance reading shown negative? - (Sep/05/2007 )
Maybe the RNA concentration is low. I will try again with another type of cuvette. Maybe my dilution also made it hard? Because I admit, I do a 50X dilution.
still depends on your concentration. Diluting 50x a concentrated solution is not a pb, but too much for low-concetrated solution.
If you have tested by electrophoresis, estimate the concentration (roughly) and calculate which dilution factor would give you a decent trustable OD