Experience with Ultramers from IDT? - Long oligos (Aug/29/2007 )
I was wondering if anyone has used Ultramers from IDT? They're long (up to 200nt) ssDNA oligos. I want to order 2 and anneal them together. Do you think annealing together such a long oligo would be problematic? And for those who have used the Ultramer, did you sequence them to see if they're correct (and were they correct?)?
If you want to build a 200 bp dsDNA fragment, then you can do this with two 110 bp fragments and doing a single no-template PCR cycle (actually I'd do a few) or anneal and extend with Klenow. The 20 bp overlap should be enough to prime either of these reactions. I would definitely expect (but hope to avoid) mutations. I haven't used the IDT oligos.