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Coupling of a peptide toCNBr-sepharose - (Jul/10/2001 )

just use the one that sigma provides with the product.



I have raised antibodies in rabbits against a peptide. To purify the antibody, I should couple the peptide to CNBr-activated sepharose. Does anybody have a detailed protocol for the coupling reaction?



go to sigma website and do a search for C9142 CNBr-activated agarose. they have a great product information sheet!!