Bright wells! - (Aug/23/2007 )

Hi all,

After I run my plasmid on an agarose gel , I see a bright shadow in wells. Is this contamination , plasmid or something else?
I load 2 ul plasmid with 3 ul loading buffer. run 1 hour in 1% agarose gel.

Thanks in Advance

-aliali-

QUOTE (aliali @ Aug 23 2007, 08:31 AM)

This is very commun, I think it’s plasmid that don’t cross.

I remember I read a post here about it long time ago with a good explanation, but I don’t remember it.

-aztecan princess-

sometimes it can be genomic DNA getting stuck in the wells.

-scolix-

for a more in-depth look, you might wish to post a picture of the gel?

thanks

-aimikins-