Renilla Luciferase Assays - Wondered if anyone knew how to make the reagents "at home"?? (Aug/14/2007 )
I am, like some here, a lowly graduate student. I therefore am not allocated much in the way of funding. I have found that the Promega Dual-Luciferase Assay (using both Firefly and Renilla) is FABULOUS....however, too costly....
Can anyone help me? I've talked the all-knowing (I joke not) post-doc into ordering Coelenterazine and Beetle Luciferin thinking this is the beginning of what I will need...but I'm not certain how much other stuff I will need to make the reagents and buffers - or what the actual reagents or buffers consist of.
Any and all help would be greatly appreciated.
As a side note...I am highly skilled at transfections with Lipofectamine in 12 different cell lines including HepG2, FAO, NIH3T3, COS-7, CHO, F9, and many others if I can be of assistance in this area...please don't hesitate to contact me or post a request.
I'd recommend looking at the patent literature. Your assay bottle may have patent numbers, or you can search the Google Patents database. For example, the Renilla assay buffer is described in patent 5,292,658. We'd probably all like to hear of your success or failure.