Collagen coverslips? - (Aug/10/2007 )
My supervisor has purchased collagen type I coverslips. Do you know of any stain we could use to check if these coverslips really contain collagen? Since it was purchased from not so well known company.
I suppose you could always buy an anti-collagen antibody but this seems like it would be a waste of money. Just try seeding some of your cells on these collagen-coated coverslip as well as normal glass coverslips. You should see a major difference in cell adherence, especially if you are working with a line that doesn't like to attach to plain glass. Might I ask where you bought these and how large are they? I work with collagen-coated coverslips which are small enough to work in 12-well plates but finding coated coverslips this small has been difficult. I currently order them from Japan which can take weeks or even months to get ahold of. It would be nice to find something else.
Might I ask why you purchased coverslips instead of collagen coating yourself? We make collagen-coated coverslips in our lab on a weekly basis - small enough to fit into a 6-well plate. It's quite a simple protocol and I could easily post it if anyone is interested. We've had great success plating primary hepatocytes on them.
Would you mind posting that. This type of question surfaces once every while. Thanks.