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Plasmid extraction from filter paper - (Aug/08/2007 )

Hi all,

I just got plasmids from our collaborator in the form of dry filter paper. Can anyone show me how to extract them out so that I can transform them into cells. Hope someone can help me here....thanks.


I used to put a tiny part of the filterpaper in a cup, add 10-50µl (depending on the size of the paper) water or TE, shake it for 5 minutes and then transformated 2µl of this into competent E.coli.



please see this recent thread :


Thanks guys