About Tween 20? - (Aug/08/2007 )
Sometimes Tween is added to BSA when blocking. Why so if BSA is already a blocking agent, just to enhance blocking of the unspecific binding or?
I do immunohistochem quite often, and I usually add Tween or Triron or both.
I fogot which (tween or triron), but one of them increase penetration of antibody and other reduce the surface tension.
I hope it help.
Tween is a polymer that blocks protein binding sites of protein binding matrices; Tween has no immunogenic potential; BSA is more complex and may bind Ab´s unspecifically (increase of background); Tween alone may not complete blocking procedure so that a mixture of different blocking agents is useful; skim milk f.i. is a natural mix of low-immunogenic proteins
(blocking solutions must always be tested for each Ab especially if there is no experience with the Ab)