kinase assay with GST-fused kinase and histone H1 as a substrate - can I try kinas assay using non-radiolabeled ATP? (Jul/24/2007 )

Hi folks,

Right now I have GST-fused kinase and H1 substrate and plan to do kinase assay. I have been searching papers about a kinase of my interest, but most of the papers used 32P isotope for kinase assay. Is it possible to do this experiment using cold ATP, do SDS-PAGE, and detect with phospho-specific H1 antibody by western blotting?

If there is someone who tried this, please give me some information about protocols etc... (eg. p-H1 antibody)

Thanks!