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Plant miRNA--Need your help! - (Jul/23/2007 )

Hi everyone

I am a beginner working on plant miRNA (oil palm miRNA). There are quite a number of posts out there on the isolation and cloning of miRNA. However there are meant for the use in animal cells. I wonder is there any difference in the protocol for animal and plant? Can I use the one for animal in plant?

I would be thankful if anyone with experience in plant miRNA study (specifically miRNA isolation and library construction) could give me some opinion/advice. Many thanks!

Nice day laugh.gif


well differences between cells and plant tissue should be the solubilization.
I guess from what i know, that liquid-nitrogen-frozen tissue should be careffully grinded and then trizol can do the job.
When you get your RNA, most of the protocols both apply in animal cells and plants.