formamide - (Jul/19/2007 )

How to use formamide to lower the Tm in the PCR

never used formamide... may you try DMSO at 0.5 1 or even 2% ?

for formamide in pcr reaction, please have a look at http://www.mcb.uct.ac.za/pcroptim.htm

2-5% Betaine has always worked best for me.