Protocol Online logo
Top : Forum Archives: : Molecular Biology

please help me to look for a red dye - (Jul/18/2007 )

Hi,everybody. Now, I am doing some lab work and need a dye: pink or scarlet in organic solvent such as phenol. Please provide me some useful infomation. Thanks!

-wuweizi-

Oil red O. I am not sure it is soluble in phenol. It is very hydrophobic, very likely it will be.

-genehunter-1-

QUOTE (genehunter-1 @ Jul 18 2007, 07:31 AM)
Oil red O. I am not sure it is soluble in phenol. It is very hydrophobic, very likely it will be.


Thanks. I try oil red in phenol. It works well. However,the color is not scarlet but dark red. I am looking for the dye:pink or scarlet in phenol.

-wuweizi-

Oxidized phenol is pink to red, so adding a red dye will mask this degradation. The oxidation products of phenol are quinones, a wide variety of phenolic coupling compounds and diacids. Quinones are a, b-unsaturated ketones that are capable of reacting with primary amines. The reaction of quinones with multiple amines can form stable crosslinked products. Quinones can crosslink nucleic acids and may break down phosphodiester bonds. The diacids arise from further oxidation of orthoquinone, while phenolic coupling products result from phenoxide radicals. Quinones are faintly yellow. Phenolic coupling reaction compounds are intense yellow or red. (1985 BRL Focus 7(2):12.)

0.1% 8-Hydroxyquinoline can be used as a preservative; this also turns the phenol layer yellow, making separation of phases easier.

-tfitzwater-