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dephosphorylated vector - (Jul/10/2007 )

How long dephosphorylated and gel eluted vector can be kept in freezer and reused for cloning.


if you did your work cleanly, especially if the DNA concentration was initially high, then I'll say the fragments should last a year, if not more. (I have an insert prep that was cut 2yrs ago and that still ligates fine. A labmate used depho vector which was 2yrs old and that ligated okay too).