Autoclave falcon tubes - (Jun/30/2007 )
i always recycle and autoclave my falcon tubes... that's what you do when you need to cut cost. the plastic won't melt with normal autoclaving process (121C for 15 min). sometimes, i even autoclave them for 45 min, if i use the tubes to grow some pathogen.
It won't melt in autoclaving process, but depends on the quality of tubes. "branded" falcon tubes, eg: falcon, granier.... can last longer -- maybe for years. but cheaper falcon tubes might not last that long and easily out of shape after several times autoclave. sometimes, you won't notice that the tubes are out of shape. but when you try to put on the cap, it doesn't fit nicely or close tighty.
if you take care of your falcon tubes... your supervisor will definately praise you for helping him to save money!!!
If I reuse my 50ml tubes even after autoclaving, my PI will have a heart attack !!!!
I think it depends on each's lab practice.. and money of course..
I don't reuse Falcon tubes in my lab (thank God that my lab is quite well-funded).
However, I heard from my seniors that they tried to autoclave the tubes but very often, the cap won't fit nicely afterwards.. and the tubes that we use are not cheap
ha, very true....
it's all about money.
but i heard from a friend, that in the lab she used to work in, they even recycle microcentrifuge tubes!!! and she was shock when she 1st work in our lab that we all throw aft used.
it's all about money.
but i heard from a friend, that in the lab she used to work in, they even recycle microcentrifuge tubes!!! and she was shock when she 1st work in our lab that we all throw aft used.
Wow... normal tubes? Is it really necessary? I mean... those tubes are pretty cheap. I will be pretty shock or perhaps worse than her if I encounter such situation.
I used to think our lab is very poor...as I am prevented from doing many experiments for my PhD thesis.
Now...I think our lab is OK...as we never recycle microcentrifuge tubes and autoclave used falcon tubes.
for DNA and RNA purpose we use new tube but for protein we re-use the tube after autoclave
Now...I think our lab is OK...as we never recycle microcentrifuge tubes and autoclave used falcon tubes.
i used to work in a lab that was so poor that we washed (manually) and reused pipet tips. of course, back then tips were a lot more expensive than they are today.
In my last lab, we did the same. Or were they only washed by the cleaning lady? I can't remember.
but in my work i used falcon tubes in doing DNA extraction. so couldn't i autoclave the tubes and reuse it???????????????????????????