Semi-dry southern blot - (Jun/26/2007 )

Hello,

I'm looking to try a semi-dry approach to southern blotting. I would like to be able to assess how sucessful the transfer of DNA to the membrane is. Does anyone know of a straight forward way to stain a membrane for DNA please?

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i think you can check it oppositely. that is after electrophoresis you take a picture of the gel. and then after blotting please take a picture again and compare the picture taken after electrophoresis and after blotting, if your DNA successfully transfered into membrane then you will find an empty gel after blotting

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Sounds more straightforward and sensible. Thanks very much

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this will just show you that the dna left the gel, not necessarily that it bound to the membrane. but, if the ethidium bromide remains intercalated with the dna then you will be able to visualize it on the membrane with a quick look with uv light.

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