Dicer knockdown in mice - (Jun/26/2007 )
Has anyone used siRNAs to silence Dicer in mouse cell lines? I’m aware of literature using this approach to knockdown Dicer in human and hamster cell lines.
Also, are there better approaches to Dicer knockdown in vitro than using siRNAs to silence a processor of siRNAs/miRNAs?
You can transiently knock down Dicer using Morpholino oligos. The following reference shows activity of Morpholinos against Dicer in zebrafish, but they are also used in cultured cells.
Wienholds E, Koudijs MJ, Van Eeden FJ, Cuppen E, Plasterk RH. The microRNA-producing enzyme Dicer1 is essential for zebrafish development. Nat Genet. 2003 Nov;35(3):217-8. Epub 2003 Oct 05.