annealing a self complementary, symmetrical oligo - (Jun/13/2007 )
I am trying to anneal a synthetic self complementary, symmetrical oligo that keeps forming a hairpin. Does anyone have any suggestions to try that can make this form a double strand?
well... i anneal previously 5-6 oligos that encode for hairpin structure after their transcription, and it's just like standard annealing.
heat in STE buffer and allow to cool. Then check on agarose gel
Just normal annealing protocol will work. we use it for shRNA stuff.