Extraction of Genomic DNA - RNase A should be added during the extraction? (Jun/11/2007 )
I m going to extract the genomic DNA from the blood samples (I will use the Qiagen kit). Should I add RNase A to digest the RNA in the extraction? Actually, the DNA samples will be used to study the mutations or SNP of certain genes. I designed the primers which flanks the exons region only. Do you think I should use the RNA free DNA samples?
Would you give me some suggestions??
Thank you so much!
I normally do not need the RNAse for blood sample DNA and SNP genotyping. I'd try it first without and if it doesn't work, you can add the RNase step.
i prefer to add RNAse after extraction of the DNA and separation with the proteins, as it uses less RNases, and the RNase itself is less sensitive to proteases.
if i use a bit higher amount of RNase, is there any chance to degrade genomic DNA???
if the RNAse is clean from DNAses, then no harm will come by using more RNAse.
However RNAse is a protein, so what goes in must then be removed.
Agree with perneseblue. Hehe... have to clean up your sample after introducing a protein in it.