# Percent (%) concentration calculations vol/vol weight/vol - (Jun/08/2007 )

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QUOTE (strawberry @ Jun 10 2007, 06:57 PM)
my god
so i made it more concentrated!!!!
thanx for explanation

you were doing it less concentrated because you were dissolving 10 mL in 100 mL (9%) which is less concentrated (because of a higher final volume) than 10 mL in 90 mL (10%). You were dissolving it too much.

-Pumuki-

QUOTE (Pumuki @ Jun 11 2007, 02:10 AM)
you were doing it less concentrated because you were dissolving 10 mL in 100 mL (9%) which is less concentrated (because of a higher final volume) than 10 mL in 90 mL (10%). You were dissolving it too much.

Strawberry, the percentage is of the final volume. So when U dissolve 10 ml in 100ml, the final volume will be 110ml. So, if you calculate 10ml of 110ml (final volume), it will be 9.09% (10/110 X 100)

Similarly, when dilutions are stated like - 1:1, 1:2, 1:4, 1:8 . . .. the second number is the final volume while the first number represents how much of the reagent/Ab do you put.

So,
1:1 will mean the substance is undiluted.
1:2 means for one part of the substance to be diluted equal volume of diluent is added (not 2 parts. here 2 = 1+1 = 1 part substance + 1 part diluent)
1:4 means for one part of the substance to be diluted you put 3 parts of diluent (not 4 parts, here 4 = 1 + 3 = 1 part of substance + 3 part diluent)
similarly
1:8 will mean 1 part of the substance and 7 parts of diluent (not 8 parts, here 8 = 1+7 = 1 part of substance + 7 part diluent)

-Nabin-

thanx, Pumuki and Nabin
it's much clear now
i used more diluted clorox then to sterilize my seeds, but hope this will not help contamination