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Blocking with 2 % BSA - (May/29/2007 )


I have found a protocol telling me that I should block my cells with 2% BSA in PBS before I perform immunocytochemistry on them. However, I do not know what 2 % exactly means in this case and the only BSA I have is in the form of a powder... I usually use goat serum and dilute it to 2% (v/v) in PBS. Does anyone have a clue?

Thanks for your replies!


2% weight/volume

or 2g in 100mL


QUOTE (beccaf22 @ May 29 2007, 02:02 PM)
2% weight/volume

or 2g in 100mL

same as becaff22, 2gms in 100ml