Western Blot Troubles - (May/29/2007 )
QUOTE (DLY @ Jun 1 2007, 02:23 PM)
Are you using a semi-dry blotter or a wet blot? Thats usually seen in semi-dry blots when the electrode plates are not entirely in contact or the papers are not soaked enough...
I use a wet-blotting system.
thanks for your comment, in future i will try to presoak the membrane for longer time. i think it could hardly be too long?!