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Western Blot Troubles - (May/29/2007 )

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QUOTE (DLY @ Jun 1 2007, 02:23 PM)
Are you using a semi-dry blotter or a wet blot? Thats usually seen in semi-dry blots when the electrode plates are not entirely in contact or the papers are not soaked enough...


I use a wet-blotting system.
thanks for your comment, in future i will try to presoak the membrane for longer time. i think it could hardly be too long?!



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