does fixation of frozen tissue sections preserve protein-protein interactions - immunohistochemistry (May/27/2007 )

I am staining frozen tissue sections with an Ab against a phosphopeptide and this Phosphate in the cell serves as docking site for the interaction with another protein. Do I expect Ab to bind for the phosphopeptide during the staining procedure or the recognition site will stay masked by the interacting protein?

Thanks

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the question can´t be answered with yes or no;

in principle, it is possible to detect phosphopeptides with an appropriate Ab in IHC; either it has been checked, f.i. by the manufacturer if it is an commercially available Ab, or oneself has to check; so the best thing is to let decide the experiment...

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