does fixation of frozen tissue sections preserve protein-protein interactions - immunohistochemistry (May/27/2007 )
I am staining frozen tissue sections with an Ab against a phosphopeptide and this Phosphate in the cell serves as docking site for the interaction with another protein. Do I expect Ab to bind for the phosphopeptide during the staining procedure or the recognition site will stay masked by the interacting protein?
the question can´t be answered with yes or no;
in principle, it is possible to detect phosphopeptides with an appropriate Ab in IHC; either it has been checked, f.i. by the manufacturer if it is an commercially available Ab, or oneself has to check; so the best thing is to let decide the experiment...