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problem is RE - (May/21/2007 )

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Hello everyone


I need help I have oligos with two RE BamH1 and Xba1 I want to clone to phCMvxi

The problem is RE in the phCMvxi the M.xba1is there any way to do the digestion with out order the M.xba1 enzyme

-biojoe-

Erm.. I dont really get you. Sorry for my English understanding. Do you mean that you have RE site of XbaI in your vector?
wink.gif

-timjim-

I will try to right it clearly I have xba1 methylated by Adenine Methylase.
I try to cut it but isn't cut is there any way to deal with it

-biojoe-

QUOTE (biojoe @ May 22 2007, 01:30 PM)
I will try to right it clearly I have xba1 methylated by Adenine Methylase.
I try to cut it but isn't cut is there any way to deal with it


do you want to cut your vector with XbaI but you cannot cut it??? do you mean that???

-T. reesei-

yes because xba1 methylated by Adenine Methylase

-biojoe-

You need to culture your plasmid in a methylase-negative E. coli strain. sad.gif

-Zouden-

transform your plasmid using a dam- competent cell

-T. reesei-

Which company you recommended to order

-biojoe-

no one Answer me sad.gif but the order it will take more time and i need it so Soon

-biojoe-

QUOTE (biojoe @ May 26 2007, 09:39 AM)
Which company you recommended to order

promega / invitrogen have them in catalog

-fred_33-

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