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Interferon - DNA sequence coding for Interferon alpha (May/19/2007 )

Hi,
I want to know that gene coding for Human Interferon alpha is NOT including exon sequences? There are only intron structures? So, to have DNA for PCR, we only need isolate DNA from blood instead mRNA?
I have not experience in this field. Can somebody help me?
Thankyou very much.

-blue molecule-

?????????? A coding gene without exons? Where should the protein come from? According to ensembl, the gene has exactly one exon, which makes it hard to design exon spanning primers. That means, that you have to be sure that NO gDNA is left in your mRNA if you are interested in the expression level of Interferon in human blood samples. (To look at the gDNA would not make too much sense as long as you are not interested in SNPs or DNA methylation).
If this doesn't answer your question, please provide more information on waht you want to do or to know.

-kr├╝melmonster-

Thankyou Krumelmonster.
I was wrong. I am isolating gene coding for human interferon alpha from blood. I start from isolating mRNA and then RT-PCR. But one my friend said that this gene has not introns. So, I only need isolate DNA directly instead mRNA.
Is this true? And where I can confirm this information?
My English is not good, can you help me?
Thankyou very much.

-blue molecule-

As mentioned above: if you are doing RT-PCR to quantify the expression, you have to stick to the mRNA. If you are doing RT PCR for any other reason (to analyze sequence variation etc.) you can use the DNA. But the latter is normally done with gDNA, so I think, if you used mRNA so far you will probably have to go on with it.
Tell me exactly what you are doing the RT-PCR for and I can tell you the correct answer. smile.gif

-kr├╝melmonster-

Thanks Krumelmonster,
I understand what you said. Do you know DNA sequence coding for human Interferon alpha does not contain introns? Is it right?And where I can confirm this? (Give me some links?)
Thanks again for your help Krumelmonster. Have fun. rolleyes.gif

-blue molecule-