SCAR-PCR - (May/18/2007 )
Just out of curiousity, does anyone of you guys know what SCAR-PCR is?
Thanks in advance
-Nanous-
I am no expert but I have a basic idea:
SCAR stands for sequence characterized amplified regions.
The idea is to design molecular probes for different strains of bacteria or funghi by first doing a PCR with a common set of primers, than sequencing the porducts and designing primers specific for the amplified regions of every strain. I think that it is mainly used for the distinguish wildtype strains of funghi or bacteria from manufactured ones (e.g. biocontrol types of Trichoderma).
Please correct me if I am completely mistaken...
-krümelmonster-