Why not dehydrate tissue in methanol before paraffin embedding? - (May/18/2007 )
I'm just learning to do embedding and sectioning, and have a general question. Most protocols tell you to dehydrate the tissue in MeOH or EtOH. If MeOH is used, then switch the sample to EtOH before going into xylene and wax. Does it make that much of a difference? Is this one of those parameters that has to be optimised with your tissue/staining method of interest?
-mirage10-
QUOTE (mirage10 @ May 18 2007, 04:41 PM)
I'm just learning to do embedding and sectioning, and have a general question. Most protocols tell you to dehydrate the tissue in MeOH or EtOH. If MeOH is used, then switch the sample to EtOH before going into xylene and wax. Does it make that much of a difference? Is this one of those parameters that has to be optimised with your tissue/staining method of interest?
of course, you may try out; EtOH is less hydrophilic than MeOH and therefore, more compatible to the following steps with hydrophobic xylene and wax...
I suppose these protocols in are most cases based on trial-and-error optimization and work in most cases
-The Bearer-