sodium Azid - concentration for ab (Nov/18/2003 )
I need to know what is the concentration of sodium azid that I should add to Ab ?
Thanks in advance
and have good day
I've always used a final concentration of 0.05% for diluted antibodies. I keep a stock of 10% NaN3 in the fridge, then use a 1:200 dilution in the final diluted Ab solution. Then I've used the antibodies 3/4 four times within 4-6 weeks. Any longer and I get different bands being recognised.