Take mice blood - (May/16/2007 )
QUOTE (Nabin @ May 27 2007, 11:16 PM)
QUOTE (vetticus3 @ May 28 2007, 12:53 PM)
oh, i've seen what you're talking about. no the eyeball is not removed. the little glass pipettes are pressed against the eye and blood is collected from the cavity. it's cruel and not the best way to collect blood. you can't collect much blood. safer and nicer methods are abound.
V
V
Vetticus, what U have seen is what Minnie has described - retroorbital method. That is not as cruel as U think it is. It is easy and safe if done the right way. Glass pippette is not pressed against the eye (which would be rather cruel) but is directed behind the eye-ball and blood is collected from the retroborbital sinus. Unless the technique is wrong damaging the optic nerve, there won't be blindness on the side of blood collection. We can collect adequate blood by this method. And, like Minnie said, if it is terminal procedure, we can do several times. The mouse is anesthetised before the procedure.
Removing eyeball is crude method of blood collection. The mouse is firmly grasped and eye ball on one side is quickly pulled out with a forcep causing it to bleed. The blood is collected in an eppendorf. Body is also massaged to get bigger drops of blood. At the end of the procedure, the mice is killed by cervical dislocation.
In my opinion, retroorbital method using the glass pippette is a good method for collection of blood from mice. What method do you use?
This is a good article to read http://www.umic.sunysb.edu/newitems/mouseblood.pdf.
Thank you Nabin!
This article is interesting as review of different blood collection methods
We collect blood from retroorbital sinus and mice feel good after this procedure but about anesthetising procedure it is not appropriate for some experiments with blood.
-circlepoint-
You can get 1-2 ml blood from a mouse through Vena Cava easily.
-genehunter-1-
Thank you Nabin for the article 
-Minnie Mouse-
QUOTE (Nabin @ May 28 2007, 05:16 PM)
That is not as cruel as U think it is. It is easy and safe if done the right way. Glass pippette is not pressed against the eye (which would be rather cruel) but is directed behind the eye-ball and blood is collected from the retroborbital sinus. ...
In my opinion, retroorbital method using the glass pippette is a good method for collection of blood from mice. What method do you use?
In my opinion, retroorbital method using the glass pippette is a good method for collection of blood from mice. What method do you use?
When i was being trained in handling animals, we had several people tell the class that this method was outdated and cruel. This was not just one person, but i can remember at least 5 people saying the same thing. if i need a bit of blood... tail vein.
then again... i'm the person who xenografted tumour cells into the mice, but because the mice were given so many treats (sunflower seeds etc), that they became fat, and at first this was mistaken for being tumour growth. d'oh.
V
-vetticus3-
QUOTE (vetticus3 @ May 28 2007, 05:18 PM)
QUOTE (Nabin @ May 28 2007, 05:16 PM)
That is not as cruel as U think it is. It is easy and safe if done the right way. Glass pippette is not pressed against the eye (which would be rather cruel) but is directed behind the eye-ball and blood is collected from the retroborbital sinus. ...
In my opinion, retroorbital method using the glass pippette is a good method for collection of blood from mice. What method do you use?
In my opinion, retroorbital method using the glass pippette is a good method for collection of blood from mice. What method do you use?
When i was being trained in handling animals, we had several people tell the class that this method was outdated and cruel. This was not just one person, but i can remember at least 5 people saying the same thing. if i need a bit of blood... tail vein.
then again... i'm the person who xenografted tumour cells into the mice, but because the mice were given so many treats (sunflower seeds etc), that they became fat, and at first this was mistaken for being tumour growth. d'oh.
V
Well! Blood collection from tail vein is good one but it is not appropriate for multiple collection ( retraction of vein after multiple collect will occur ) of blood samples to study dynamic processes in blood or serum composition in some exp like LCS
-circlepoint-
QUOTE (circlepoint @ May 29 2007, 12:45 AM)
QUOTE (vetticus3 @ May 28 2007, 05:18 PM)
QUOTE (Nabin @ May 28 2007, 05:16 PM)
That is not as cruel as U think it is. It is easy and safe if done the right way. Glass pippette is not pressed against the eye (which would be rather cruel) but is directed behind the eye-ball and blood is collected from the retroborbital sinus. ...
In my opinion, retroorbital method using the glass pippette is a good method for collection of blood from mice. What method do you use?
In my opinion, retroorbital method using the glass pippette is a good method for collection of blood from mice. What method do you use?
When i was being trained in handling animals, we had several people tell the class that this method was outdated and cruel. This was not just one person, but i can remember at least 5 people saying the same thing. if i need a bit of blood... tail vein.
then again... i'm the person who xenografted tumour cells into the mice, but because the mice were given so many treats (sunflower seeds etc), that they became fat, and at first this was mistaken for being tumour growth. d'oh.
V
Well! Blood collection from tail vein is good one but it is not appropriate for multiple collection ( retraction of vein after multiple collect will occur ) of blood samples to study dynamic processes in blood or serum composition in some exp like LCS
Tail vein bleeding is a really good option--we take blood from the tail vein on a weekly basis for about 6 months and have not had any problems with veins retracting. I think it you do it properly (and carefully) you will not damage the tail in any way.
-mouser-
QUOTE (mouser @ Jun 6 2007, 12:58 PM)
QUOTE (circlepoint @ May 29 2007, 12:45 AM)
QUOTE (vetticus3 @ May 28 2007, 05:18 PM)
QUOTE (Nabin @ May 28 2007, 05:16 PM)
That is not as cruel as U think it is. It is easy and safe if done the right way. Glass pippette is not pressed against the eye (which would be rather cruel) but is directed behind the eye-ball and blood is collected from the retroborbital sinus. ...
In my opinion, retroorbital method using the glass pippette is a good method for collection of blood from mice. What method do you use?
In my opinion, retroorbital method using the glass pippette is a good method for collection of blood from mice. What method do you use?
When i was being trained in handling animals, we had several people tell the class that this method was outdated and cruel. This was not just one person, but i can remember at least 5 people saying the same thing. if i need a bit of blood... tail vein.
then again... i'm the person who xenografted tumour cells into the mice, but because the mice were given so many treats (sunflower seeds etc), that they became fat, and at first this was mistaken for being tumour growth. d'oh.
V
Well! Blood collection from tail vein is good one but it is not appropriate for multiple collection ( retraction of vein after multiple collect will occur ) of blood samples to study dynamic processes in blood or serum composition in some exp like LCS
Tail vein bleeding is a really good option--we take blood from the tail vein on a weekly basis for about 6 months and have not had any problems with veins retracting. I think it you do it properly (and carefully) you will not damage the tail in any way.
Hi mouser!
Thank you for comments!
I think you are right , because we inject substances in tail vein between blood collections so it is possible that this substance had retraction effect
-circlepoint-