Take mice blood - (May/16/2007 )
Is there anyone having an experience in collecting mice blood by removing the whole eye ball? Is it ethical? and how much blood can be obtained?
Thanks in advance!
I have seen others performing the orbital sinus bleeding but not by removing the whole eye ball. In fact, this is the first time that I ever heard about this method. Does it really exist?
I don't remember how much of blood that we can obtain from the orbital sinus bleeding. Perhaps you can check the literature if you want to get the answer
remove the eyeball? if you are going to sacrifice the mice, just do a heart stick - you can get about 600ul if you're competent, and sometimes a little more. it just takes practice. why would you want to remove the eyeball?
If it is a terminal procedure, you may bleed from the reto-orbital and get a mL. If it is not a terminal procedure, you may get the 1% of the body weight as desired. I have never heard of removing the eyeball itself--this is not necessary and you certainly wouldn't want to do it without it being terminal and without the mouse being in the surgical plane of anesthesia.
Cardiac sticks are good for terminal procedures, too. You can get a least a mL if it's an adult mouse.
Other ways to bleed on non-terminal procedures are sapehnous vein and tail vein.
Thanks in advance!
I 've never heard about this technic ( eye ball) . We collect blood from ROS by paster pipette ( near 0.4-0.5 ml ( we obtain 0.1-0.2ml serum) and mice feel well after this procedure. Cardiac punction - 0,5ml blood and high skilled person to do it. Cutting edge of tail - 0.5ml.
I do not think you can collect blood from the mice by removing his eyes.
The orbital sinus bleeding. This technique has nothing to do with the eye ball and it does not affect the eye at all.
It gives you blood more than the tail. I did try this method with rats and you can fill the whole plain tube with blood if you want. But If you do not want to kill the animal take the amount of blood that you need only because this might cause anemia.
The animal needs two weeks to recover after collecting the blood by this method.
Yes, you can collect blood by removing the eyeball with forceps. But, I am not sure how ethical that is. The amount is also not so impressive. may be just around .75 to 1 ml.
Is it done by the way anywhere?
Just curious 
.
May I ask why do you need to remove the mouse's eyeball in order to take its blood??
Are you planning to kill it after taking its blood?
If you need more blood, why not bleed it twice by reto-orbital..
once 0.5ml...twice 1ml....or even three times 1.5ml of blood
Then you will have... once 0.2ml...twice 0.4ml or even three times 0.6ml of serum.
oh, i've seen what you're talking about. no the eyeball is not removed. the little glass pipettes are pressed against the eye and blood is collected from the cavity. it's cruel and not the best way to collect blood. you can't collect much blood. safer and nicer methods are abound.
V
V
Vetticus, what U have seen is what Minnie has described - retroorbital method. That is not as cruel as U think it is. It is easy and safe if done the right way. Glass pippette is not pressed against the eye (which would be rather cruel) but is directed behind the eye-ball and blood is collected from the retroborbital sinus. Unless the technique is wrong damaging the optic nerve, there won't be blindness on the side of blood collection. We can collect adequate blood by this method. And, like Minnie said, if it is terminal procedure, we can do several times. The mouse is anesthetised before the procedure.
Removing eyeball is crude method of blood collection. The mouse is firmly grasped and eye ball on one side is quickly pulled out with a forcep causing it to bleed. The blood is collected in an eppendorf. Body is also massaged to get bigger drops of blood. At the end of the procedure, the mice is killed by cervical dislocation.
In my opinion, retroorbital method using the glass pippette is a good method for collection of blood from mice. What method do you use?
This is a good article to read http://www.umic.sunysb.edu/newitems/mouseblood.pdf.