Where exactly does a restriction enzyme cut in its recognition site? - (May/07/2007 )
Restriction enzyme cut site is described cut within the nucleotides..say for eg....in CCGGA it is CCG-GA. But imagine the nucleotide structure....Sugar-Phosphate-Nucleotide base....does it cleave the hydrogen bond..??? or the sugar phosphate bond..or the phosphate- nucleotide bond..if dephosphorylation works....ppbly it is the phosphate-nucleotide??? Is tht right???
Restriction enzymes hydrolyze the backbone of DNA between deoxyribose and phosphate groups. This leaves a phosphate group on the 5' ends and a hydroxyl on the 3' ends of both strands