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Where exactly does a restriction enzyme cut in its recognition site? - (May/07/2007 )

Restriction enzyme cut site is described cut within the nucleotides..say for CCGGA it is CCG-GA. But imagine the nucleotide structure....Sugar-Phosphate-Nucleotide base....does it cleave the hydrogen bond..??? or the sugar phosphate bond..or the phosphate- nucleotide bond..if dephosphorylation works....ppbly it is the phosphate-nucleotide??? Is tht right???


Restriction enzymes hydrolyze the backbone of DNA between deoxyribose and phosphate groups. This leaves a phosphate group on the 5' ends and a hydroxyl on the 3' ends of both strands