Protocol Online logo
Top : Forum Archives: : Immunology and Histology

Control for HIF-1alpha staining? - (Apr/19/2007 )

Hi there,

I'm planning to do immunohistochemistry on hypoxia marker, HIF-1alpha. To may knowledge, the positive control for this marker is mamalian cells under reduced oxygen level.

Anyone knows what are the other controls which can be used for HIF-1alpha?

Thanks in advance!

Farizan.

-Farizan-

Cartilage is hypoxic in vivo, and uses HIF mediated transcrition for controlling a number of critical functions

-JAH-