multiplex reverse transcription - (Apr/16/2007 )
I noticed that someone else already proposed this question, though no answers were given. I was wondering whether it is possible to do a multiplex reverse transcription reaction on rna samples, and than use these samples in a ' normal' real-time pcr analysis. Can the results actually be interpreted as quantitative? Has anyone found reports/publications that actually propose this method, or varify it?
Thanks a lot ,
I think the answer is no, how can you control for differing efficiencies of each primer in the RT reaction?
That is exactly one of my concerns... the thing is, i've tested these primers on more pure samples, and there the efficiencies of all the primers are very much the same.... (though not exact; so i'd guess that if they were different 5%, it would be reproduced twice now instead on only once)