Protocol Online logo
Top : Forum Archives: : Molecular Biology

Do restriction enzymes work on dUTP? - (Mar/30/2007 )

I have a question,
Can restriction enzymes cut ds DNAs whose dTTPs are substituted by dUTPs?
And what about if substitution occurs on one strand only?



Personally, I would doubt it, as t he purpose of the enzyme is to get rid of foreign DNA. Tell you what, try it, and see what happens. It'll only take a morning to do the digest and then run a quick gel. But I wouldn't be betting anything of value on the outcome...